The oppD Gene and Putative Peptidase Genes May Be Required for Virulence in Mycoplasma gallisepticum.
Identifieur interne : 000184 ( Main/Exploration ); précédent : 000183; suivant : 000185The oppD Gene and Putative Peptidase Genes May Be Required for Virulence in Mycoplasma gallisepticum.
Auteurs : Chi-Wen Tseng [Australie] ; Chien-Ju Chiu [Australie] ; Anna Kanci [Australie] ; Christine Citti [France] ; Renate Rosengarten [Autriche] ; Glenn F. Browning [Australie] ; Philip F. Markham [Australie]Source :
- Infection and immunity [ 1098-5522 ] ; 2017.
Descripteurs français
- KwdFr :
- Animaux, Facteurs de virulence (génétique), Infections à Mycoplasma (génétique), Infections à Mycoplasma (microbiologie), Maladies de la volaille (génétique), Maladies de la volaille (microbiologie), Mutagenèse par insertion (génétique), Mycoplasma gallisepticum (génétique), Mycoplasma gallisepticum (pathogénicité), Poulets (microbiologie), Protéines bactériennes (génétique), Protéines bactériennes (métabolisme), Virulence (génétique).
- MESH :
- génétique : Facteurs de virulence, Infections à Mycoplasma, Maladies de la volaille, Mutagenèse par insertion, Mycoplasma gallisepticum, Protéines bactériennes, Virulence.
- microbiologie : Infections à Mycoplasma, Maladies de la volaille, Poulets.
- métabolisme : Protéines bactériennes.
- pathogénicité : Mycoplasma gallisepticum.
- Animaux.
English descriptors
- KwdEn :
- Animals, Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Chickens (microbiology), Mutagenesis, Insertional (genetics), Mycoplasma Infections (genetics), Mycoplasma Infections (microbiology), Mycoplasma gallisepticum (genetics), Mycoplasma gallisepticum (pathogenicity), Poultry Diseases (genetics), Poultry Diseases (microbiology), Virulence (genetics), Virulence Factors (genetics).
- MESH :
- chemical , genetics : Bacterial Proteins, Virulence Factors.
- chemical , metabolism : Bacterial Proteins.
- genetics : Mutagenesis, Insertional, Mycoplasma Infections, Mycoplasma gallisepticum, Poultry Diseases, Virulence.
- microbiology : Chickens, Mycoplasma Infections, Poultry Diseases.
- pathogenicity : Mycoplasma gallisepticum.
- Animals.
Abstract
Relatively few virulence genes have been identified in pathogenic mycoplasmas, so we used signature-tagged mutagenesis to identify mutants of the avian pathogen Mycoplasma gallisepticum with a reduced capacity to persist in vivo and compared the levels of virulence of selected mutants in experimentally infected chickens. Four mutants had insertions in one of the two incomplete oppABCDF operons, and a further three had insertions in distinct hypothetical genes, two containing peptidase motifs and one containing a member of a gene family. The three hypothetical gene mutants and the two with insertions in oppD1 were used to infect chickens, and all five were shown to have a reduced capacity to induce respiratory tract lesions. One oppD1 mutant and the MGA_1102 and MGA_1079 mutants had a greatly reduced capacity to persist in the respiratory tract and to induce systemic antibody responses against M. gallisepticum The other oppD1 mutant and the MGA_0588 mutant had less capacity than the wild type to persist in the respiratory tract but did elicit systemic antibody responses. Although M. gallisepticum carries two incomplete opp operons, one of which has been acquired by horizontal gene transfer, our results suggest that one of the copies of oppD may be required for full expression of virulence. We have also shown that three hypothetical genes, two of which encode putative peptidases, may be required for full expression of virulence in M. gallisepticum. None of these genes has previously been shown to influence virulence in pathogenic mycoplasmas.
DOI: 10.1128/IAI.00023-17
PubMed: 28348054
Affiliations:
- Australie, Autriche, France
- Midi-Pyrénées, Occitanie (région administrative), Victoria (État), Vienne (Autriche)
- Melbourne, Toulouse, Vienne (Autriche)
- Université de Melbourne
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Le document en format XML
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Markham</name><affiliation wicri:level="4"><nlm:affiliation>Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author></analytic><series><title level="j">Infection and immunity</title><idno type="eISSN">1098-5522</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Bacterial Proteins (genetics)</term><term>Bacterial Proteins (metabolism)</term><term>Chickens (microbiology)</term><term>Mutagenesis, Insertional (genetics)</term><term>Mycoplasma Infections (genetics)</term><term>Mycoplasma Infections (microbiology)</term><term>Mycoplasma gallisepticum (genetics)</term><term>Mycoplasma gallisepticum (pathogenicity)</term><term>Poultry Diseases (genetics)</term><term>Poultry Diseases (microbiology)</term><term>Virulence (genetics)</term><term>Virulence Factors (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Facteurs de virulence (génétique)</term><term>Infections à Mycoplasma (génétique)</term><term>Infections à Mycoplasma (microbiologie)</term><term>Maladies de la volaille (génétique)</term><term>Maladies de la volaille (microbiologie)</term><term>Mutagenèse par insertion (génétique)</term><term>Mycoplasma gallisepticum (génétique)</term><term>Mycoplasma gallisepticum (pathogénicité)</term><term>Poulets (microbiologie)</term><term>Protéines bactériennes (génétique)</term><term>Protéines bactériennes (métabolisme)</term><term>Virulence (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>Virulence Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Bacterial Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Mutagenesis, Insertional</term><term>Mycoplasma Infections</term><term>Mycoplasma gallisepticum</term><term>Poultry Diseases</term><term>Virulence</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Facteurs de virulence</term><term>Infections à Mycoplasma</term><term>Maladies de la volaille</term><term>Mutagenèse par insertion</term><term>Mycoplasma gallisepticum</term><term>Protéines bactériennes</term><term>Virulence</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Infections à Mycoplasma</term><term>Maladies de la volaille</term><term>Poulets</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Chickens</term><term>Mycoplasma Infections</term><term>Poultry Diseases</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines bactériennes</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Mycoplasma gallisepticum</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Mycoplasma gallisepticum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Relatively few virulence genes have been identified in pathogenic mycoplasmas, so we used signature-tagged mutagenesis to identify mutants of the avian pathogen Mycoplasma gallisepticum with a reduced capacity to persist in vivo and compared the levels of virulence of selected mutants in experimentally infected chickens. Four mutants had insertions in one of the two incomplete oppABCDF operons, and a further three had insertions in distinct hypothetical genes, two containing peptidase motifs and one containing a member of a gene family. The three hypothetical gene mutants and the two with insertions in oppD1 were used to infect chickens, and all five were shown to have a reduced capacity to induce respiratory tract lesions. One oppD1 mutant and the MGA_1102 and MGA_1079 mutants had a greatly reduced capacity to persist in the respiratory tract and to induce systemic antibody responses against M. gallisepticum The other oppD1 mutant and the MGA_0588 mutant had less capacity than the wild type to persist in the respiratory tract but did elicit systemic antibody responses. Although M. gallisepticum carries two incomplete opp operons, one of which has been acquired by horizontal gene transfer, our results suggest that one of the copies of oppD may be required for full expression of virulence. We have also shown that three hypothetical genes, two of which encode putative peptidases, may be required for full expression of virulence in M. gallisepticum. None of these genes has previously been shown to influence virulence in pathogenic mycoplasmas.</div></front></TEI><affiliations><list><country><li>Australie</li><li>Autriche</li><li>France</li></country><region><li>Midi-Pyrénées</li><li>Occitanie (région administrative)</li><li>Victoria (État)</li><li>Vienne (Autriche)</li></region><settlement><li>Melbourne</li><li>Toulouse</li><li>Vienne (Autriche)</li></settlement><orgName><li>Université de Melbourne</li></orgName></list><tree><country name="Australie"><region name="Victoria (État)"><name sortKey="Tseng, Chi Wen" sort="Tseng, Chi Wen" uniqKey="Tseng C" first="Chi-Wen" last="Tseng">Chi-Wen Tseng</name></region><name sortKey="Browning, Glenn F" sort="Browning, Glenn F" uniqKey="Browning G" first="Glenn F" last="Browning">Glenn F. Browning</name><name sortKey="Chiu, Chien Ju" sort="Chiu, Chien Ju" uniqKey="Chiu C" first="Chien-Ju" last="Chiu">Chien-Ju Chiu</name><name sortKey="Kanci, Anna" sort="Kanci, Anna" uniqKey="Kanci A" first="Anna" last="Kanci">Anna Kanci</name><name sortKey="Markham, Philip F" sort="Markham, Philip F" uniqKey="Markham P" first="Philip F" last="Markham">Philip F. Markham</name></country><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Citti, Christine" sort="Citti, Christine" uniqKey="Citti C" first="Christine" last="Citti">Christine Citti</name></region></country><country name="Autriche"><region name="Vienne (Autriche)"><name sortKey="Rosengarten, Renate" sort="Rosengarten, Renate" uniqKey="Rosengarten R" first="Renate" last="Rosengarten">Renate Rosengarten</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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